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170 er nk1 1  (fluidigm)


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    fluidigm 170 er nk1 1
    a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and <t>CD19−NK1.1−CD11b−CD11c−TCRβ+</t> (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.
    170 Er Nk1 1, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/170 er nk1 1/product/fluidigm
    Average 95 stars, based on 57 article reviews
    170 er nk1 1 - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Clonally expanded CD8 T cells characterize Amyotrophic Lateral Sclerosis 4"

    Article Title: Clonally expanded CD8 T cells characterize Amyotrophic Lateral Sclerosis 4

    Journal: Nature

    doi: 10.1038/s41586-022-04844-5

    a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.
    Figure Legend Snippet: a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.

    Techniques Used: Mass Cytometry, Expressing, Flow Cytometry



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    a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and <t>CD19−NK1.1−CD11b−CD11c−TCRβ+</t> (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.
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    a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and <t>CD19−NK1.1−CD11b−CD11c−TCRβ+</t> (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.
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    a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and <t>CD19−NK1.1−CD11b−CD11c−TCRβ+</t> (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.
    170 Er, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/170 er/product/fluidigm
    Average 95 stars, based on 1 article reviews
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    Image Search Results


    a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.

    Journal: Nature

    Article Title: Clonally expanded CD8 T cells characterize Amyotrophic Lateral Sclerosis 4

    doi: 10.1038/s41586-022-04844-5

    Figure Lengend Snippet: a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.

    Article Snippet: The following isotope conjugated anti-mouse antibodies were used at 1:100 dilution: 113 In CD45.1 (clone A20, eBioscience, Catalog # 14–0453-85), 115 In CD45.2 (clone 104, eBioscience, Catalog # 14–0454-85), 141 Pr Ly6G (clone 1A8, Fluidigm, Catalog # 3141008B), 142 Nd CD11c (clone N418, Biolegend, Catalog # 117302), 143 Nd TCRbeta (clone H57–597, Fluidigm, Catalog # 3143010B), 144 Nd CD24 (clone M1/69, Biolegend, Catalog # 101802),145 Nd CD69 (clone H1.2F3, Fluidigm, Catalog # 3145005B), 146 Nd F4/80 (clone BM8, Fluidigm), 147 Sm CD357/GITR (clone DTA-1, Biolegend), 148 Nd CD11b (clone M1/70, Biolegend, Catalog #), 149 Sm CD19 (clone 6D5, Fluidigm, Catalog #), 150 Nd IgD (clone 11–26c.2a, Biolegend, Catalog #), 151 Eu CD25 (clone 3C7, Fluidigm, Catalog # 3146008B), 152 Sm SiglecF (clone S17007L, Biolegend, Catalog # 155502), 153 Eu NKp46 (clone 29A1.4, Fluidigm, Catalog # 3153006B), 155 Gd CD73 (clone TY/11.8, Biolegend, Catalog # 127202), 156 Gd CD64 (clone X54–5/7.1, Biolegend, Catalog # 139302), 158 Gd CD117 (clone 2B8, Biolegend, Catalog # 105802), 159 Tb PD-1 (clone RMP1, Biolegend, Catalog #), 160 Gd CD62L (clone MEL-14, Fluidigm, Catalog # 109113),162 Dy Ly6C (clone HK1.4, Biolegend, Catalog # 128002),163 Dy anti-APC (Biolegend, Catalog # 408002),164 Dy Sca-1 (clone D7, Fluidigm, Catalog # 3164005B), 165 Ho anti-PE (Biolegend, Catalog # 408102), 166 Er KLRG1 (clone 2F1/KLRG1, Biolegend, Catalog # 93135),167 Er CXCR3 (clone CXCR3–173, Biolegend, Catalog # 126526), 168 Er CD8alpha (clone 53–6.7, Biolegend, Catalog # 100702), 169 Tm CD206 (clone C068C2, Fluidigm, Catalog # 3169021B), 170 Er NK1.1 (clone PK136, Fluidigm, Catalog # 3170002B), 171 Yb CD44 (clone IM7, Biolegend, Catalog # 103002), 172 Yb CD4 (clone RM4–5, Fluidigm, Catalog # 3172003B), 174 Yb MHCII (I-A/I-E) (clone M5/114.15.2, Biolegend, Catalog # 107602), 175 Lu CD127 (clone A7R34, Biolegend, Catalog # 135002), 176 Yb B220 (RA3–6B2, clone Biolegend, Catalog # 103202).

    Techniques: Mass Cytometry, Expressing, Flow Cytometry